[11] Xenotransplantation, N 5, september 2003, Glasgow, UK

Influence of Pluripotent Progenitor Embryonic Cells

on the Transplant Against the Host Reaction by

Bone Marrow Xenotransplantation in the Experiment.

Cells, Embryo, Nobe Marrow, Transplantation, Proteolysis

Kukharchuk. A: Radchenko. V; Sirman, V

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Embryonic pluripotent progenitor cells (EPPC) have been isolated from the rat's embryos on the 13th day of pregnancy. It has been examined the EPPC influence on the transplant against the host reaction intensity by bone marrow transplantation in nils. Bone marrow was extracted from femurs by washing with the use of DMEM medium and has been carried out intraperitoneal administration in the dosage of 8 x 10⁶ ml. The first group of rats - before bone marrow transplantation - it has been injected EPPC in the dosage of 2 x 10⁷ ml. The second group of rats - it has been injected Hanks solution. Bone marrow xenotransplantation has been carried out on the basis of immune depression (cyclosporine A, L-asparaginase). In one month in rats of the control group has been established the significant enhancement of the proteolytic proteins destruction (albumin-, casein-, and collagenolytic activity) in the bowels, skin, thymus, spleen, lymph nodes and bone marrow tissues. In the rats which have been inserted EPPC the proteolysis intensity was equal lo such as in the intact animals. It has been established the fact of fixation of EPPC marked by PKH-67 in the thymus and bone marrow. In the immune system organs after the EPPC administration has been discovered the increase of the cells number with the typical morphological signs of apoptosis, and also activity increase of caspasc-8, c tspase-3.