[41] Journal of artificial organs, N 4, Vol.28, 2005, Milano-Birminghem-Osaka,

Embryonic pluripotent progenitor cells (EPPC)

induce the central immunological tolerance in rats and mice

O. Kukharchuk. V. Radchenkо, V. Sirman

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Administration of the cells from transplanted organ into recipient's thymus facilitates the engraftment under the mechanisms of T-lymphocytes negative selection. However, such strategy requires an additional operation and does not completely replace the immunosuppression in postoperative period, as the central tolerance is limited in age of the cells induced in thymus, presenting the foreign antigens and MHC molecules. To study a possibility of long term central tolerance induction the engraftment of skin and spleen allograft after intravenous EPPC administration in large doses in rats was studied. Methods: PKH-67 staining was used to identify EPPC in thymus. Results of skin and spleen allotransplantation were evaluated by engraftment.

Results: EPPC administration in large doses, performed simultaneously with skin and spleen transplantation, facilitates a long term engraftment. In thymus of experimental animals the cells marked with PKH-67 were found. Conclusions: EPPC intravenous administration in large doses, performed simultaneously with skin and spleen allotransplantation, can induce the central immunological tolerance without donor antigens administration of transplanted organs in thymus.

Keywords: Engraftment, Immunological tolerance, Thymus, Donor antigen, Immunosuppression.